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Molecule List for Accession CaMKII_2003 (Accession Number49)

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The entries are grouped according to Pathway Number and are alternately color coded using

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color.

	Name	Pathway Name / Pathway No.	Accession Type	Initial Conc. (uM)	Volume (fL)	Buffered	Sum Total Of
1	CaMKII	CaMKII Pathway No. 202	Network	70	1000	No	-
	Huge conc of CaMKII. In PSD it is 20-40% of protein, so we assume it is around 2.5% of protein in spine as a whole. This level is so high it is unlikely to matter much if we are off a bit. This comes to about 70 uM.
2	CaM	CaM Pathway No. 203	Network	20	1000	No	-
	There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels.
3	neurogranin	CaM Pathway No. 203	Network	10	1000	No	-
	Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding ....
4	PP1-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	1.8	1000	No	-
	Cohen et al Meth Enz 159 390-408 is main source of info conc = 1.8 uM
5	I1	PP1 Pathway No. 204	Network	1.8	1000	No	-
	I1 is a 'mixed' inhibitor, but at high enz concs it looks like a non-compet inhibitor (Foulkes et al Eur J Biochem 132 309-313 9183). We treat it as non-compet, so it just turns the enz off without interacting with the binding site. Cohen et al ann rev bioch refer to results where conc is 1.5 to 1.8 uM. In order to get complete inhib of PP1, which is at 1.8 uM, we need >= 1.8 uM.
6	CaNAB	PP2B Pathway No. 205	Network	1	1000	No	-
	We assume that the A and B subunits of PP2B are always bound under physiol conditions. Up to 1% of brain protein = 25 uM. I need to work out how it is distributed between cytosolic and particulate fractions. Tallant and Cheung '83 Biochem 22 3630-3635 have conc in many species, average for mammalian brain is around 1 uM.
7	PP2A	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.12	1000	No	-
8	PKC-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.09	1000	Yes	-
9	Ca	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.08	1000	Yes	-
10	PKA-active	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0.015	1000	Yes	-
11	I1*	PP1 Pathway No. 204	Network	0.001	1000	No	-
	Dephosph is mainly by PP2B
12	CaM-Ca4	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-
13	CaMKII-CaM	CaMKII Pathway No. 202	Network	0	1000	No	-
14	CaMKII-thr286*-C aM	CaMKII Pathway No. 202	Network	0	1000	No	-
	From Hanson and Schulman, the thr286 is responsible for autonomous activation of CaMKII.
15	CaMKII***	CaMKII Pathway No. 202	Network	0	1000	No	-
	From Hanson and Schulman, the CaMKII does a lot of autophosphorylation just after the CaM is released. This prevents further CaM binding and renders the enzyme quite independent of Ca.
16	CaMKII-thr286	CaMKII Pathway No. 202	Network	0	1000	No	-
	I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off.
17	CaMK-thr306	CaMKII Pathway No. 202	Network	0	1000	No	-
	This forms due to basal autophosphorylation, but I think it has to be considered as a pathway even if some CaM is floating around. In either case it will tend to block further binding of CaM, and will not display any enzyme activity. See Hanson and Schulman JBC 267:24 pp17216-17224 1992
18	neurogranin-CaM	CaM Pathway No. 203	Network	0	1000	No	-
19	neurogranin*	CaM Pathway No. 203	Network	0	1000	No	-
	The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993)
20	CaM-Ca3	Shared_Object_ CaMKII_2003 Pathway No. 201	Network	0	1000	No	-

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